Application of PCR genotyping of bacterial isolates in turkey breeding

Abstract

Scientific research on the identification of genetic resistance determinants
of the microflora of turkeys using primers in 2020 - 2021, the SGC “North Caucasus Zonal
Experimental Station of Poultry”of the Stavropol Territory, together with the Department of
Microbiology of All-Russian Research Veterinary Institute of Poultry Farming and the Laboratory of Molecular Genetics of All-Russian Research Institute of Genetics and Farm Animal
Breeding, carried out a work, which was aimed at creating a reliable protection of the turkey
livestock from bacterial infections on an industrial scale. The work is based on genotyping
and PCR methods to identify genes that provide antibiotic resistance in bacterial isolates of
turkeys. As a result, the genomic DNA of E. coli bacteria was revealed. The fact of widespread antibiotic resistance in this group of bacteria was established. The genetic resistance
determinants in the turkey microflora were identified. A variant of the veterinary prevention
measures scheme was created. The PCR genotyping method for bacterial isolates is recommended for use in poultry farms.