DETERMINING THE OPTIMAL NOISE EXPOSURE DURATION TO INDUCE OXIDATIVE STRESS IN LABORATORY ANIMAL MODELS

Abstract

In order to simulate oxidative stress in a warm-blooded organism, the
prooxidant effects of noise were tested with daily exposure of 30, 60, and 90 minutes for 21
days. The study was conducted at the central research laboratory of the Amur Medical
Academy (Blagoveshchensk) in 2023–2024. The experiment involved 120 albino male rats
weighing 190 – 240g, divided into four groups. The control group of animals received no
noise exposure, while the first, second, and third experimental groups were exposed to noise
daily for three weeks for 30, 60, and 90 minutes, respectively. The results of the experiment
revealed that modeling oxidative stress in laboratory animals through noise exposure reveals a
tendency toward a prooxidant shift in equilibrium with daily noise exposure for 30 minutes
over 21 days. With an increase in the duration of exposure to acoustic load to 60 minutes per
day, a statistically significant accumulation was observed compared to the intact group of
diene conjugates (by 31% on days 7 and 14 of the experiment, 48% on day 21, p < 0,05), lipid
hydroperoxides (by 39% on days 7 and 14 of the experiment, 42% on day 21, p < 0,05),
malondialdehyde (by 48% on day 7, 44% on day 14, 64% on day 21, p < 0,05). At the same
time, the activity of ceruloplasmin (by 45%, 48% and 44%, respectively, p < 0.05) and vitamin E decreased (by 26% on day 14, 29% on day 21, p < 0,05). Increasing the duration of
daily noise exposure to 90 minutes also contributes to the formation of oxidative stress with a
reliable accumulation of lipid peroxidation products and depletion of the antioxidant system
by the end of the first, second and third weeks of the experiment, however, the absence of
statistically significant differences between the second and third experimental groups does not
allow us to establish benefits in terms of changing the concentration of prooxidant and
antioxidant markers of oxidative stress, which justifies the possibility of recommending a
daily 60-minute acoustic load to create a model of oxidative stress in vivo.