ИЗУЧЕНИЕ ТОКСИЧНОСТИ КОРМОВОЙ ДОБАВКИ АА-50 НА ЛАБОРАТОРНЫХ ЖИВОТНЫХ

Abstract

The article presents the results of the study of an irritant effect, acute and
chronic toxicity of the dietary feed additive AA-50. Feed additive AA-50 is a liquid based on
whey and lemon balm decoction, which contains a microbial mass of live natural strains of
microorganisms Bacillus subtilis and Bacillus licheniformis in an optimal ratio. Before using
AA-50 feed additive in poultry feeding, it is necessary to determine its safety on laboratory
animals. The experiments to determine the parameters of acute and chronic toxicity of AA-50
feed additive were carried out on outbred white rats and mice. The irritant effect of the feed
additive was determined on albino rabbits using the skin application method. The experiments
to determine the toxicity of AA-50 feed additive on laboratory mice were carried out in the
vivarium of the Faculty of Veterinary Medicine in Kuban State Agrarian University named
after. I. T. Trubilin. When determining acute toxicity, laboratory animals received the feed
additive orally through a single intragastric administration. Mice received 0,5; 0.4; and 0,3 ml
of feed additive. As for rats, they received 5,0; 4,0 and 3,0 ml. The general condition of the
animals was satisfactory. When determining the chronic toxicity, laboratory animals received
the feed additive intragastrically for 30 days: mice were administered 0,5; 0,4; and 0,3 ml of
feed additive. As for rats, they received 5,0; 4,0 and 3,0 ml. There were no deviations in the
behavior of animals. During the pathomorphological autopsy, it was noted that the location of
internal organs of white mice and white rats of the experimental groups was morphologically
correct. When analyzing the body weight of laboratory animals, it was found that the animals
of the experimental groups slightly exceeded this parameter of the intact group. The effect of
the feed additive on the metabolism of laboratory animals was assessed by morphological and
biochemical blood values. All studied blood values were within physiological values. When
determining the irritant effect of AA-50 feed additive using the cutaneous application method,
no positive skin reactions were detected, which led to the conclusion that the primary irritation index was 0. The results, which were obtained when assessing the acute and chronic tox icity of AA-50 feed additive in recommended dosages, did not have a toxic effect on the body
of laboratory animals.