RAPD-PCR TECHNIQUE FOR GENOTYPING OF TURKEY BACTERIAL ISOLATES

Abstract

Decree of the Government of the Russian Federation dated 25 August, 2017
No. 996 “On approval of the Federal scientific and technical program for the development of
agriculture for 2017-2025” determines the research of the SGC “North Caucasus ZESP”,
which was aimed at preventing and control of infectious bacterial diseases of birds, which
cause huge economic damage to the industry. This study corresponds to the tasks, which were
set by the Integrated Target Program of the Russian Federation in the priority area “Poultry
farming”. The creation of domestic competitive lines and crosses of turkeys requires a reliable
system of livestock protection, which can be based on the use of modern molecular and genetic techniques, including genotyping and PCR tests for antibiotic resistance of pathogenic microorganisms. The studies are based on the use of the genotyping method of pathogenic microorganisms in bacteriology, using PCR to detect the presence of genes that determine antibiotic resistance in microorganisms. The use of modern techniques of genotyping makes it
possible to solve epizootological issues. In 2022, at the production base of the SGC “North
Caucasus ZESP”, samples were taken from the mortality of turkeys for RAPD-PCR genotyping – a fast technique based on the use of random short primers ERIC 1, 2 and M13. Cultures
of bacterial isolates were obtained in differential diagnostic media to confirm the species identity. Genomic DNA was isolated from bacteria of the species Escherichia coli. A search for
antibiotic resistance genes in this type of turkey pathogen was carried out. The fact of widespread antibiotic resistance in the studied group of bacterial isolates was established. This
technique made it possible to correct the scheme of veterinary preventive measures for turkeys and is recommended for use in turkey farms.