Age-related activity of superoxide dismutase in horses, dogs and goats

Abstract

A study was conducted at the St. Petersburg State University of Veterinary
Medicine in order to study the age-related features of superoxide dismutase activity (EC
1.15.1.1.) of clinically healthy horses, dogs and goats. The study material was blood of
clinically healthy animals. SOD activity was determined in clinically healthy domestic horses
(Equusferuscaballus) of the Latvian breed, domestic goats (Capra aegagrus hircus) of the
Saanen breed, domestic dogs (Canis lupus familiaris) of the German Shepherd breed. The
animals were divided into 5 age groups. Domestic goat: Group I – 1 year old, group II – 2
years old, group III – 3 years old, group IV – 4 years old, group V – 5 years old. Domestic
horse: Group I – 1 year old, group II – 1-3 years old, group III – 3-5 years old, group IV – 5-
10 years old, group V – 10-15 years old. Domestic dog: Group I – 1-2 years old, group II – 3-
4 years old, group III – 5-6 years old, group IV – 7-8 years old, group V – older than 9 years
old. The activity of superoxide dismutase was determined by the method of the recovery
inhibition of nitroblue tetrazolium with the presence of NADH2. As a result of the study, SOD
activity was established for goats, horses and dogs for each age group. The maximum activity
of SOD for dogs and goats was recorded in group III and it is 23.5±0.2 U/mg of protein and
20.1±1.8 U/mg of protein, respectively. For horses, the period of maximum SOD activity is
observed in the age group V – it is 20.1±0.3 U/mg of protein. The average age-related activity
of SOD was determined. For goats, the average age-related activity of SOD is equal to 16.3 ±
0.75 U/mg of protein. For horses, the activity is 16.96±0.74 U/mg of protein. For dogs, the
average age-related activity of SOD is 16.84±1.18 U/mg of protein.