Development of technology for obtaining ointment composition based on colloidal zinc oxide for veterinary science

Abstract

Within the context of the article, а study of the effectiveness of zinc oxide
as a component of a wound healing ointment was carried out. Nanoscale zinc oxide was
obtained by sol-gel method in an alcohol medium. The structure was studied with the help of
scanning electron microscopy by a Mira-LMH scanning electron microscope (“Tescan”,
Czech Republic). The obtained scanning electron microscope (SEM) images showed that the
synthesized ZnO nanoparticles had a quasi-spherical shape and an average size of 30 nm. The
research was conducted on the effectiveness of healing of burn wounds on 60 white male
Wistar rats, whose mass was 170 ± 20 g. Laboratory rats were divided into 3 groups. Within
the model experiment, burn wounds of the 1st group of rats were treated with a developed
ointment composition, which is based on zinc oxide nanoparticles. Burn wounds of the 2nd
group were treated with commercial zinc ointment. The 3rd group was control. The obtained
data showed that the area of burn wounds decreased throughout the experiment in each test
group of laboratory rats. As a result of the experiments, it was found that the ointment
composition, which was based on zinc oxide nanoparticles, had a pronounced regenerative
effect on a burn wound, including comparison with the existing analogue of commercial zinc
ointment. When treating with ointment, which was based on ZnO nanoparticles, the rate of
reduction of burn wounds was 5 times higher in comparison to the 3rd group and 1.5 times in
comparison to the 2nd group of rats. It was found that the developed ointment composition,
which was based on zinc oxide nanoparticles, showed a regenerative effect 1 day after its
application and had a healing degree 27.5% higher in comparison to the 2nd group and 32.1%
in comparison to the control group.